|
|
| Cellufine Ion Exchangers are based
on spherical particles manufactured from crosslinked cellulose. Each
offers excellent flow properties, mechanical stability and
chemical resistance. These ion exchangers are ideally suited
for both laboratory and process scale chromatography of proteins, peptides
and other biomolecules. Applications include the purification
of antibodies, growth factors, albumin, enzymes, nucleic
acids, etc. |
Features
• Spherical particles exhibiting high mechanical strength
• Choice of several pore sizes
• Hydrophilic
• DEAE (A), QA (Q) anion exchange and carboxylmethyl
(C) cation exchange
• Pre-swollen
• Virtually no shrinkage or swelling
• Resistant to 0.5M NaOH
• Stable in organic solvents
• Autoclavable (121 °C for 30 minutes)
• Passes USP VI acute systemic implant tests |
Benefits
• High flow rates allowing rapid chromatography and
direct scale-up
• Allows optimum capacity matched to molecular weight
• Low non-specific binding
• Optimal functionality selection
• Easy packing
• Easy large scale operation
• Easy regeneration and depyrogenation
• Allows alternative cleaning regimes
• Sterilizable
• Non toxic
• Regulatory support |
Partial Structure
 |
|
|
| Type |
A-200
Weak Anion |
A-500
Weak Anion |
A-800
Weak Anion |
Q-500
Strong Anion |
C-500
Weak Cation |
| Functional
Group |
DEAE |
DEAE |
DEAE |
QA |
CM |
| Basic Matrix |
Spherical , Crosslinked
Cellulose Beads |
| Particle
Size |
44
–105 µm |
53
–125 µm |
53
–125 µm |
53
–125 µm |
53
–125 µm |
| Exclusion Limit
(kD) |
>30 |
>500 |
>1,000 |
>500 |
>500 |
| pH
Working Range |
2
– 12 |
| pH Stability
Range |
1 –
14 |
| Operating
Pressure |
Up
to 2 bar (29 psi) |
| Shrinkage/Swelling |
<5 % on
change
from H2O to
0.5M NaCl |
Negligible |
Negligible |
Negligible |
Negligible |
| Supplied |
Suspension
in 20 % EtOH |
|
|
Titration Curves
 |
Adsorption Capacity
| |
Ion |
Protein
(mg/ml) |
| Media |
(meq/g)
dry |
BSA |
Lysozyme |
| A-200 |
0.9 |
>80 |
– |
| A-500 |
1.3 |
>60 |
– |
| A-800 |
0.8 |
>45 |
– |
| Q-500 |
1.5 |
>10 |
– |
| C-500 |
1.1 |
– |
>70 |
|
Flow Properties
Pressure flow curves for Cellufine Ion Exchangers in laboratory
and process scale columns.
|
Mechanical Resistance
Cellufine Ion Exchangers are semi-rigid due to the crosslinked
structure which gives high mechanical resistance and results in
two advantages; no generation of fines during the preparation
of the column and higher operating bed height without concern
of bed collapse common to many ‘soft gels’. |
Chemical Resistance
Cellufine is chemically stable. The media can be washed with
a variety of solutions and shows no loss in capacity, resolution
or flow rates.
| Solvent |
Exposure
without loss
at room temperature |
| Methanol |
Compatible |
| Ethanol |
Compatible |
| 6M Guanidine HCI |
Compatible |
| 8M Urea |
Compatible |
| 1% Triton X |
Compatible |
| 1M Acetic Acid |
7 days |
| 0.5M NaOH |
30 days |
0.5M NaOH
+1M NaCl
|
30 days |
| 0.1M HCl |
30 days |
|
Applications
|
|
|
| Description |
Quantity |
Catalogue
No. |
| Cellufine
A-200 |
100
ml
500 ml
5 liters
10 liters |
6769
803 27
19611
19612
6769 803 35 |
| Cellufine A-500 |
100 ml
500 ml
5 liters
10 liters |
6759 803 27
19805
19806
6759 803 35 |
| Cellufine
A-800 |
100
ml
500 ml
5 liters
10 liters |
6739
803 27
19800
19801
6739 803 35 |
| Cellufine Q-500 |
100 ml
500 ml
5 liters
10 liters |
6759 823 27
19907
19908
6759 823 35 |
| Cellufine C-500 |
100
ml
500 ml
5 liters
10 liters |
6759
833 27
19865
19866
6759 833 35 |
|
|
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