|
|
Gel Filtration, or size exclusion chromatography, has
proven to be a simple and effective purification method for proteins
and other biomolecules. However, the traditionally slow flow rates
and limiting resolving power of most gel filtration media have
restricted its usefulness. These problems, inherent to the packings,
existed not only at the laboratory preparative scale but especially
at process scale. The limited throughput of gel filtration columns
has often created a significant process bottleneck.
Cellufine GCL media overcome these limitations. The mechanical
strength of the spherical cellulose matrix allows high flow rates,
even in large industrial columns. |
Features
• Rigid spherical particles
• Outstanding mechanical strength
• Hydrophilic
• High pore volume
• Broad selection of fractionation range
• Supplied preswollen
• High chemical resistance
• Autoclavable at 121 °C for 30 minutes |
Benefits
• High flow rate compatibility even in large diameter process
columns
• May be pumped or stirred without fines generation
• Low non-specific adsorption and high recovery
• Good capacity
• Optimize selectivity to required molecular weight range
• No time-consuming swelling procedures required
• Usable with all common salts, detergents, chaotropic agents,
organic solvents
• Depyrogenation
• Sterilizable
• Regulatory Support |
|
|
|
Matrix |
Cross linked cellulose |
| Particle
Shape |
Spherical |
| Particle
Size |
GCL-2000, 53 –
125 µm |
| Chemical
Resistance |
Media can be used
with most salts, detergents, solvents, acids and bases,
including 8M urea, 6M guanidine / HCL, 0.1M HCL and
0.5M NaOH |
| Mechanical
Resistance |
Media will withstand
peristaltic pumping and extended magnetic stirring |
| Schrinkage
/ Swelling |
Shrinkage or swelling < 3 % with changes in
pH or ionic strength. Shrinkage or swelling with
solvents varies with solvent and media
|
| Autoclavable |
121 °C for 30
minutes |
| Supplied |
Suspension in 20
% ethanol |
|
|
Range of Fractionalization
This media offer an extraordinarily broad selection of fractionation
ranges: from the unique Cellufine GCL-2000 for very high molecular
weight protein complexes. Table 1 shows the exclusion limit for
the media. |
 |
| Cellufine
Packing |
Exclusion
Limit (Daltons) |
| PEG |
Proteins |
Polysaccharides |
| GCL-2000 |
200,000 |
3,000,000 |
— |
|
|
| Selectivity |
| Protein |
Daltons |
Kav |
Thyroglobulin
Ferritin
Catalase
lgG
Aldolase
Alcohol-Dehydrogenase
Bovine Serum Albumun
Ovalbumin
ß-Lactoglobulin
Chymotrypsinogen A
Myoglobin
Ribonuclease
Cytochrome C
Insulin B-Chain
Bacitracin
(Glycine)4
Glycine |
660,000
440,000
210,000
160,000
58,000
150,000
67,000
46,000
42,000
25,000
17,000
14,000
12,500
3,500
1,400
246
75 |
0.18
0.25
0.33
0.33
0.35
0.35
0.39
0.47
0.51
0.60
0.60
0.65
0.67
—
0.90
—
— |
|
|
Pressure Flow Curves
 |
Protein Recovery
| Protein |
%
Recovery |
| Catalase |
100 |
| lgG |
100 |
| Aldolase |
100 |
| Serum Albumin |
100 |
| Lactoglobulin |
100 |
| Ribonuclease |
100 |
| Cytochrome C |
100 |
| Lysozyme |
100 |
| Chymotrypsinogen A |
99 |
| Myoglobin |
97 |
|
|
|
| Description |
Quantity |
Catalogue
No. |
Cellufine
GCL-2000 |
100
ml
0.5 L
5 L
10 L |
6720
003 27
19791
19792
6720 003 35 |
|
|
|
inquiry
